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Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009 Apr 12.

Photoconversion in orange and red fluorescent proteins.

Author information

1
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.

Abstract

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

PMID:
19363494
PMCID:
PMC2675661
DOI:
10.1038/nmeth.1319
[Indexed for MEDLINE]
Free PMC Article

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