Format

Send to

Choose Destination
Int J Hematol. 2009 May;89(4):497-507. doi: 10.1007/s12185-009-0303-8. Epub 2009 Apr 11.

Clinical significance of co-expression of CD21 and LFA-1 in B-cell lymphoma.

Author information

1
Department of Bioregulatory Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, 791-0295, Japan.
2
Cancer Center, Ehime University Graduate School of Medicine, Toon, Ehime, 791-0295, Japan. yoshiyak@m.ehime-u.ac.jp.
3
Department of Pathology, Kurume University School of Medicine, Fukuoka, Japan.
4
Department of Pathology, Ehime University Graduate School of Medicine, Ehime, Japan.
5
Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan.
6
Division of Blood Transfusion and Cell Therapy, Ehime University Graduate School of Medicine, Ehime, Japan.
7
Department of Bioregulatory Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, 791-0295, Japan. yasukawa@m.ehime-u.ac.jp.

Abstract

We previously reported that the prognosis of CD21-positive diffuse large B-cell lymphoma (DLBCL) is significantly favorable to that of CD21-negative DLBCL (Otsuka et al. in Br J Haematol 127:416-424, 2004). In this study, we attempted to clarify the biological significance of CD21 expression in B-cell lymphoma (BCL) by performing in vitro experiments using CD21 transfection into a CD21-negative lymphoma cell line and analyzing clinical data from lymphoma samples. Established clones of CD21 transfectants showed homotypic aggregation in suspension culture. Analysis of integrin expression revealed that LFA-1 appeared to be expressed on CD21 transfectants, and the cell aggregation was abrogated by anti-LFA-1 antibody. The CD21 transfectants could adhere to plastic plates coated with ICAM-1. Moreover, flow cytometry and/or immunohistochemical analyses of clinical BCL samples (n = 29) revealed positive for CD21 in all cases; LFA-1 was also expressed without exception. All BCL cells isolated from cavity fluids (n = 10) failed to express both CD21 and LFA-1. These data suggest that CD21 is tightly related to LFA-1 expression in BCL and the absence of CD21/LFA-1 expression is associated with pleural/peritoneal fluid involvement by BCL, a potential indicator of disease progression of BCL.

PMID:
19360456
DOI:
10.1007/s12185-009-0303-8
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center