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Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7251-6. doi: 10.1073/pnas.0900952106. Epub 2009 Apr 9.

Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination.

Author information

1
RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Erratum in

  • Proc Natl Acad Sci U S A. 2009 May 26;106(21):8791.

Abstract

In higher plants, the circadian clock controls a wide range of cellular processes such as photosynthesis and stress responses. Understanding metabolic changes in arrhythmic plants and determining output-related function of clock genes would help in elucidating circadian-clock mechanisms underlying plant growth and development. In this work, we investigated physiological relevance of PSEUDO-RESPONSE REGULATORS (PRR 9, 7, and 5) in Arabidopsis thaliana by transcriptomic and metabolomic analyses. Metabolite profiling using gas chromatography-time-of-flight mass spectrometry demonstrated well-differentiated metabolite phenotypes of seven mutants, including two arrhythmic plants with similar morphology, a PRR 9, 7, and 5 triple mutant and a CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1)-overexpressor line. Despite different light and time conditions, the triple mutant exhibited a dramatic increase in intermediates in the tricarboxylic acid cycle. This suggests that proteins PRR 9, 7, and 5 are involved in maintaining mitochondrial homeostasis. Integrated analysis of transcriptomics and metabolomics revealed that PRR 9, 7, and 5 negatively regulate the biosynthetic pathways of chlorophyll, carotenoid and abscisic acid, and alpha-tocopherol, highlighting them as additional outputs of pseudo-response regulators. These findings indicated that mitochondrial functions are coupled with the circadian system in plants.

PMID:
19359492
PMCID:
PMC2667372
DOI:
10.1073/pnas.0900952106
[Indexed for MEDLINE]
Free PMC Article

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