Format

Send to

Choose Destination
Drug Metab Lett. 2009 Jan;3(1):45-53.

Impact of time-dependent inactivation on the estimation of enzyme kinetic parameters for midazolam.

Author information

1
Pharmacokinetics, Dynamics, and Drug Metabolism, Pfizer Global Research and Development, Groton, Connecticut 06340, USA. Craig.Giragossian@Pfizer.com

Abstract

Enzyme kinetic parameters for midazolam were time-dependent in human liver microsomes, under initial velocity conditions. V(max) and K(m) decreased up to 3.7 and 3.1-fold, respectively, for 10 min compared to 1 min incubations. Mathematical models describing the relationship between inactivation and the time-dependency of enzyme kinetic parameter estimates were derived and discussed.

PMID:
19356116
DOI:
10.2174/187231209787176353
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center