Abstract
Rat liver microsomes attached to nanoparticles were used for LC-MS studies of CYP3A and 2E1 enzymes in metabolism of N-nitroso compounds. Using these biocolloids, turnover rates were measured within 2 min. Inhibitor IC(50) values for ketoconazole (KET) and 4-methylpyrazole (4-MEP) were estimated.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Chromatography, Liquid / methods
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Colloids / chemistry
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Cytochrome P-450 CYP2E1 / metabolism*
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Cytochrome P-450 CYP2E1 Inhibitors
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Cytochrome P-450 CYP3A / metabolism*
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Cytochrome P-450 CYP3A Inhibitors
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Enzyme Inhibitors / administration & dosage
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Enzyme Inhibitors / pharmacology*
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Fomepizole
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Inhibitory Concentration 50
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Ketoconazole / administration & dosage
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Ketoconazole / pharmacology
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Microsomes, Liver / enzymology
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Microsomes, Liver / metabolism
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Nanoparticles
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Nitroso Compounds / metabolism*
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Pyrazoles / administration & dosage
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Pyrazoles / pharmacology
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Rats
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Tandem Mass Spectrometry / methods
Substances
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Colloids
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Cytochrome P-450 CYP2E1 Inhibitors
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Cytochrome P-450 CYP3A Inhibitors
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Enzyme Inhibitors
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Nitroso Compounds
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Pyrazoles
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Fomepizole
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Cytochrome P-450 CYP2E1
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Cytochrome P-450 CYP3A
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Ketoconazole