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Lab Chip. 2009 Apr 21;9(8):1033-6. doi: 10.1039/b820985e. Epub 2009 Mar 3.

Controlling the selection stringency of phage display using a microfluidic device.

Author information

1
Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

PMID:
19350081
DOI:
10.1039/b820985e
[Indexed for MEDLINE]

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