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Nat Methods. 2009 May;6(5):347-9. doi: 10.1038/nmeth.1316. Epub 2009 Apr 6.

Single molecule-sensitive probes for imaging RNA in live cells.

Author information

1
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. philip.santangelo@bme.gatech.edu

Abstract

To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.

PMID:
19349979
PMCID:
PMC4297622
DOI:
10.1038/nmeth.1316
[Indexed for MEDLINE]
Free PMC Article

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