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J Med Primatol. 2009 Aug;38(4):272-8. doi: 10.1111/j.1600-0684.2009.00353.x. Epub 2009 Mar 31.

Surface phenotype and rapid quantification of blood dendritic cell subsets in the rhesus macaque.

Author information

1
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, and Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Abstract

BACKGROUND:

The study of dendritic cell (DC) biology in the rhesus macaque is becoming increasingly important but is limited by incomplete characterization and the lack of a rapid assay to quantify cells.

METHODS:

We characterized the surface phenotype of myeloid (mDC) and plasmacytoid DC (pDC) subsets in healthy rhesus macaque blood and developed a flow cytometry-based assay for absolute DC determinations.

RESULTS:

Rhesus CD11c(+) mDC were CD16(+) CD11b(+) CD56(lo) CD8(-) CD1c(-) whereas CD123(+) pDC lacked expression of these markers. Precise DC determinations were performed using a rapid two-step assay combining the analysis of whole blood and peripheral blood leukocytes (PBL).

CONCLUSIONS:

Antibodies to CD11b, CD56 and CD16 must be omitted from the lineage antibody cocktail to prevent inadvertent gating-out of DC when analyzing rhesus blood. The combined whole-blood/PBL quantification assay will be invaluable for the rapid and repeated monitoring of blood DC counts in this species.

PMID:
19344375
PMCID:
PMC3073502
DOI:
10.1111/j.1600-0684.2009.00353.x
[Indexed for MEDLINE]
Free PMC Article

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