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Angew Chem Int Ed Engl. 2009;48(18):3362-5. doi: 10.1002/anie.200900337.

A single residue influences the reaction mechanism of ammonia lyases and mutases.

Author information

1
Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Strasse 4, 17487 Greifswald, Germany.

Erratum in

  • Angew Chem Int Ed Engl. 2010 May 25;49(23):3860.

Abstract

All ways lead to Rome? Computer modeling and kinetic measurements identified a distinct residue in Phe/Tyr ammonia lyases (PAL/TAL) which controls whether the Friedel-Crafts or an E(1)cB reaction mechanism takes place. Hence, Glu484 in pcPAL favors the Friedel-Crafts reaction (see picture, MIO = 4-methylidene imidazol-5-one) whereas an Asn in TAL gives an elimination reaction. These mechanistic investigations also reveal activity of a PAL mutant and a TAL towards an amino alcohol.

PMID:
19343746
DOI:
10.1002/anie.200900337
[Indexed for MEDLINE]

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