Format

Send to

Choose Destination
Org Biomol Chem. 2009 Apr 21;7(8):1705-8. doi: 10.1039/b900233b. Epub 2009 Mar 12.

Glycosylation engineering of spinosyn analogues containing an L-olivose moiety.

Author information

1
Biotica Technology Ltd, Chesterford Research Park, Cambridge, UK CB10 1XL.

Abstract

Biosynthetic genes encoding proteins involved in the first steps of deoxyhexose biosynthesis from D-glucose-1-phosphate were expressed in Saccharopolyspora erythraea. The resulting mutant was able to accumulate and utilise TDP-L-olivose. Co-expression of the spinosyn glycosyl transferase SpnP in the resulting mutant endowed upon it the ability to biotransform exogenously added spinosyn aglycones to yield novel spinosyn analogues.

PMID:
19343260
DOI:
10.1039/b900233b
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Royal Society of Chemistry
Loading ...
Support Center