Analysis of cyclosporin A in dried blood spots using liquid chromatography tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1595-8. doi: 10.1016/j.jchromb.2009.03.024. Epub 2009 Mar 24.

Abstract

Dried blood spot sampling is a promising and patient friendly alternative for venous sampling. A liquid chromatography tandem mass spectrometer assay was developed for analyzing cyclosporin A in dried blood spots. Linearity ranged from 25 to 1440 microg/L. Within and between run accuracy and precision were within limits. The developed assay has a negligible matrix-effect and a recovery of 97%. The dried blood spots were stable during a period of at least 17 days in the refrigerator. The developed assay is suitable for analyzing cyclosporin A in dried blood spots.

Publication types

  • Evaluation Study

MeSH terms

  • Chromatography, Liquid / methods*
  • Cyclosporine / blood*
  • Humans
  • Specimen Handling
  • Tandem Mass Spectrometry / methods*

Substances

  • Cyclosporine