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J Microbiol Methods. 2009 Jun;77(3):323-5. doi: 10.1016/j.mimet.2009.03.009. Epub 2009 Mar 31.

One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae.

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1
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

Abstract

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.

PMID:
19341768
DOI:
10.1016/j.mimet.2009.03.009
[Indexed for MEDLINE]

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