Format

Send to

Choose Destination
See comment in PubMed Commons below
Appl Opt. 2009 Apr 1;48(10):D79-87.

Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy.

Author information

1
Department of Chemistry, University of California, Irvine, California 92697, USA.

Abstract

Second-harmonic generation (SHG) imaging is combined with coherent anti-Stokes Raman scattering (CARS) microscopy to follow the process of optical clearing in human skin ex vivo using dimethyl sulfoxide (DMSO) as the optical clearing agent. SHG imaging revealed that DMSO introduces morphological changes to the collagen I matrix. By carefully measuring the dynamic tissue attenuation of the coherent nonlinear signal, using CARS reference signals during the clearing process, it is found that DMSO reduces the overall SHG response from dermal collagen. Evidence is provided for a role of DMSO in compromising the structure of collagen fibers, associated with a reduction of the tissue's scattering properties.

PMID:
19340127
PMCID:
PMC2803712
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America Icon for PubMed Central
    Loading ...
    Support Center