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PLoS One. 2009;4(3):e5030. doi: 10.1371/journal.pone.0005030. Epub 2009 Mar 31.

Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility.

Author information

1
Department of Cell Biology, Nijmegen Centre for Molecular Life Sciences (NCMLS), Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.

Abstract

BACKGROUND:

Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocytotic activity of macrophages, processes sharing dependency on actomyosin dynamics.

METHODOLOGY/PRINCIPAL FINDINGS:

Here, we provide evidence for direct coupling between CK-B and actomyosin activities in cortical microdomains of astrocytes and fibroblasts during spreading and migration. CK-B transiently accumulates in membrane ruffles and ablation of CK-B activity affects spreading and migration performance. Complementation experiments in CK-B-deficient fibroblasts, using new strategies to force protein relocalization from cytosol to cortical sites at membranes, confirmed the contribution of compartmentalized CK-B to cell morphogenetic dynamics.

CONCLUSION/SIGNIFICANCE:

Our results provide evidence that local cytoskeletal dynamics during cell motility is coupled to on-site availability of ATP generated by CK-B.

PMID:
19333390
PMCID:
PMC2659440
DOI:
10.1371/journal.pone.0005030
[Indexed for MEDLINE]
Free PMC Article

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