Format

Send to

Choose Destination
Mitochondrion. 2009 Jul;9(4):254-60. doi: 10.1016/j.mito.2009.03.004. Epub 2009 Mar 28.

Mutations in the heme b-binding residue of SDHC inhibit assembly of respiratory chain complex II in mammalian cells.

Author information

1
Imperial College London, Department of Experimental Medicine and Toxicology, Division of Investigative Science, Du Cane Road, London W12 0NN, UK.

Abstract

Respiratory chain complex II has been extensively studied but little is known about its assembly and the role of its heme group. Mutations in the phylogenetically conserved histidine 127 of the SDHC subunit have been shown to abrogate heme binding in yeast and bacteria without impairing complex II assembly or enzymatic activities. Here we show that in mammalian cells these mutations lead to a complete reduction of SDHC in mitochondria, a destabilisation of SDHD and SDHB, and to an abrogation of complex II enzymatic activities, suggesting that in mammalian cells complex II assembly is more complex than in lower organisms.

PMID:
19332149
DOI:
10.1016/j.mito.2009.03.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center