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Appl Environ Microbiol. 2009 Jun;75(11):3826-33. doi: 10.1128/AEM.02542-08. Epub 2009 Mar 27.

Hollow-fiber membrane chamber as a device for in situ environmental cultivation.

Author information

1
Waseda Institute for Advanced Study, Waseda University, Shinjuku-ku, Tokyo, Japan. yoshiteruaoi@aoni.waseda.jp

Abstract

A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

PMID:
19329655
PMCID:
PMC2687273
DOI:
10.1128/AEM.02542-08
[Indexed for MEDLINE]
Free PMC Article
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