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J Med Chem. 2009 Apr 23;52(8):2192-5. doi: 10.1021/jm900201b.

Development of an in-capillary approach to nanoscale automated in vitro cytochromes p450 assays.

Author information

1
School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.

Abstract

A method to perform nanoscale automated CYP450-based drug metabolism studies using a capillary as a reaction vessel is described. In-capillary assays consumed only approximately 30 nL of recombinant human CYP450 solution. Ultrafast analysis of substrates and metabolites was achieved off-line by ultrahigh-pressure liquid chromatography coupled to mass spectrometry. This approach was successfully applied to qualitative metabolism studies of six major CYP450 isozymes and CYP2D6 inhibition experiments.

PMID:
19326942
DOI:
10.1021/jm900201b
[Indexed for MEDLINE]

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