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Biotechniques. 2009 Feb;46(2):130-2. doi: 10.2144/000113066.

High-throughput verification of transcriptional starting sites by Deep-RACE.

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1
Functional Genomics Technology Team, Omics Science Center, RIKEN Yokohama Institute, Kanagawa, Japan.

Abstract

We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

PMID:
19317658
DOI:
10.2144/000113066
[Indexed for MEDLINE]
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