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Biotechniques. 1991 Aug;11(2):226-31.

Antibody quantitation in seconds using affinity Perfusion Chromatography.

Author information

1
PerSeptive Biosystems, Inc., University Park at MIT, Cambridge, MA 02139.

Abstract

An extremely rapid assay technique for antibodies has been developed utilizing protein A or protein G bound to Perfusion Chromatography support matrices. Either dilute or concentrated samples are directly injected on a column that selectively binds antibody, which is quantitated directly by elution and UV absorbance. Due to the unique mass transport characteristics of the supports, total assay cycle times are typically 1 minute or less, with assays as short as 15 seconds possible. The assay system can accurately quantitate a 100,000:1 or greater dynamic range in sample concentration without sample dilution, is extremely repeatable and is easy to automate with conventional HPLC systems. Assay of antibodies in a wide range of sample types has been demonstrated.

PMID:
1931021
[Indexed for MEDLINE]

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