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Appl Environ Microbiol. 2009 May;75(10):3352-4. doi: 10.1128/AEM.02407-08. Epub 2009 Mar 20.

Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.

Author information

1
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, NSW 2052, Australia. b.ferrari@unsw.edu.au

Abstract

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

PMID:
19304839
PMCID:
PMC2681634
DOI:
10.1128/AEM.02407-08
[Indexed for MEDLINE]
Free PMC Article
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