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J Urol. 2009 May;181(5):2332-8. doi: 10.1016/j.juro.2009.01.014. Epub 2009 Mar 19.

Tamm-horsfall protein protects against urinary tract infection by proteus mirabilis.

Author information

1
Department of Medicine/Nephrology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.

Abstract

PURPOSE:

Proteus mirabilis is a common cause of urinary tract infection. We determined the role of Tamm-Horsfall protein as a host defense factor against the cystitis and pyelonephritis caused by P. mirabilis.

MATERIALS AND METHODS:

We generated Tamm-Horsfall protein gene knockout mice using homologous recombination. We introduced P. mirabilis transurethrally into the bladder of Tamm-Horsfall protein deficient (THP(-/-)) and genetically similar WT (THP(+/+)) mice. We cultured urine to quantitate the degree of bacteriuria. We examined bladders and kidneys grossly and histomorphometrically to determine the intensity of inflammation.

RESULTS:

THP(-/-) mice had more severe bacteriuria and cystitis than THP(+/+) mice. THP(-/-) mice had more pyelonephritic abscesses than THP(+/+) mice. The severity of histological pyelonephritis on semiquantitative histomorphometric analysis appeared to be greater in THP(-/-) mice. The difference between the 2 groups approached but did not attain statistical significance (p = 0.053).

CONCLUSION:

Tamm-Horsfall protein acts as a host defense factor against P. mirabilis induced urinary tract infection.

PMID:
19303096
PMCID:
PMC2930622
DOI:
10.1016/j.juro.2009.01.014
[Indexed for MEDLINE]
Free PMC Article

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