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Curr Opin Biotechnol. 2009 Feb;20(1):14-8. doi: 10.1016/j.copbio.2009.02.013. Epub 2009 Mar 18.

Engineered luciferases for molecular sensing in living cells.

Author information

1
Promega Corporation, Madison, WI 53711, USA.

Abstract

As a means for visualizing molecular physiology within living cells, new strategies are emerging for engineering luciferases into intracellular biosensors. These biosensors can be classified as bimolecular, relying on complementation of luciferase fragments, or intramolecular, relying on domain insertion within the luciferase structure. Multiple design strategies have recently surfaced for the development of intramolecular sensors, allowing dynamic detection of small molecules or post-translational modifications within cells. Building on successes achieved in cell culture, these sensors are now beginning to reveal molecular processes within living organisms.

PMID:
19299118
DOI:
10.1016/j.copbio.2009.02.013
[Indexed for MEDLINE]

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