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Anal Chim Acta. 2009 Apr 6;638(1):51-7. doi: 10.1016/j.aca.2009.01.063. Epub 2009 Feb 8.

Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment.

Author information

1
Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria. alexander.leitner@univie.ac.at

Abstract

Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.

PMID:
19298879
DOI:
10.1016/j.aca.2009.01.063
[Indexed for MEDLINE]

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