Abstract
As nonenveloped viruses, the aquareoviruses and orthoreoviruses are unusual in their ability to induce cell-cell fusion and syncytium formation. While an extraordinary family of fusion-associated small transmembrane (FAST) proteins is responsible for orthoreovirus syncytiogenesis, the basis for aquareovirus-induced syncytiogenesis is unknown. We now report that the S7 genome segment of an Atlantic salmon reovirus is polycistronic and uses a noncanonical CUG translation start codon to produce a 22-kDa integral membrane protein responsible for syncytiogenesis. The aquareovirus p22 protein represents a fourth distinct member of the FAST family with a unique repertoire and arrangement of structural motifs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Chlorocebus aethiops
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Genome, Viral / genetics
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Giant Cells / cytology*
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Giant Cells / metabolism*
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Membrane Proteins / chemistry
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Membrane Proteins / classification
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Open Reading Frames / genetics
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Protein Biosynthesis / genetics*
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Reoviridae / genetics
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Reoviridae / metabolism*
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Salmo salar / virology
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Viral Proteins / chemistry
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Viral Proteins / classification
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Viral Proteins / genetics
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Viral Proteins / metabolism*
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Virus Internalization
Substances
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Membrane Proteins
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Viral Proteins
Associated data
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GENBANK/FJ652575
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GENBANK/FJ652576