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PLoS One. 2009;4(3):e4894. doi: 10.1371/journal.pone.0004894. Epub 2009 Mar 17.

Human TRIM gene expression in response to interferons.

Author information

1
Département des Maladies Infectieuses, Institut Cochin, Université Paris Descartes, CNRS, UMR 8104, Paris, France.

Abstract

BACKGROUND:

Tripartite motif (TRIM) proteins constitute a family of proteins that share a conserved tripartite architecture. The recent discovery of the anti-HIV activity of TRIM5alpha in primate cells has stimulated much interest in the potential role of TRIM proteins in antiviral activities and innate immunity.

PRINCIPAL FINDINGS:

To test if TRIM genes are up-regulated during antiviral immune responses, we performed a systematic analysis of TRIM gene expression in human primary lymphocytes and monocyte-derived macrophages in response to interferons (IFNs, type I and II) or following FcgammaR-mediated activation of macrophages. We found that 27 of the 72 human TRIM genes are sensitive to IFN. Our analysis identifies 9 additional TRIM genes that are up-regulated by IFNs, among which only 3 have previously been found to display an antiviral activity. Also, we found 2 TRIM proteins, TRIM9 and 54, to be specifically up-regulated in FcgammaR-activated macrophages.

CONCLUSIONS:

Our results present the first comprehensive TRIM gene expression analysis in primary human immune cells, and suggest the involvement of additional TRIM proteins in regulating host antiviral activities.

PMID:
19290053
PMCID:
PMC2654144
DOI:
10.1371/journal.pone.0004894
[Indexed for MEDLINE]
Free PMC Article

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