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Bioorg Med Chem. 2009 Apr 1;17(7):2859-63. doi: 10.1016/j.bmc.2009.02.024. Epub 2009 Feb 20.

Incorporation of an inducible nucleotide analog into DNA by DNA polymerases.

Author information

1
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Canada ON L8N 3Z5.

Abstract

Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.

PMID:
19286386
DOI:
10.1016/j.bmc.2009.02.024
[Indexed for MEDLINE]

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