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Biochem Biophys Res Commun. 2009 Mar 13;380(3):460-6. doi: 10.1016/j.bbrc.2009.01.113. Epub 2009 Jan 24.

Ptpcd-1 is a novel cell cycle related phosphatase that regulates centriole duplication and cytokinesis.

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1
Department of Cell Biology and Biochemistry, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

Abstract

Proper progression of mitosis requires spatio-temporal regulation of protein phosphorylation by orchestrated activities of kinases and phosphatases. Although many kinases, such as Aurora kinases, polo-like kinases (Plks), and cyclin B-Cdk1 are relatively well characterized in the context of their physiological functions at mitosis and regulation of their enzymatic activities during mitotic progression, phosphatases involved are largely unknown. Here we identified a novel protein tyrosine phosphatase containing domain 1 (Ptpcd 1) as a mitotic phosphatase, which shares sequence homology to Cdc14. Immunofluorescence studies revealed that Ptpcd1 partially colocalized with gamma-tubulin, an archetypical centrosomal marker. Overexpression of this phosphatase prevented unscheduled centrosomal amplification in hydroxyurea arrested U2OS cells. Intriguingly, Ptpcd 1-associated and colocalized with polo-like kinase 1(Plk1). Hence, overexpression of Ptpcd1 rescued prometaphase arrest of Plk-1 depleted cells, but resulted in aberrant cytokinesis as did as Plk1 overexpression. These results suggested that Ptpcd1 is involved in centrosomal duplication and cytokinesis.

PMID:
19284988
DOI:
10.1016/j.bbrc.2009.01.113
[Indexed for MEDLINE]
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