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Curr Protoc Cell Biol. 2009 Mar;Chapter 3:Unit 3.32. doi: 10.1002/0471143030.cb0332s42.

Isolation of dense core secretory vesicles from pancreatic endocrine cells by differential and density gradient centrifugation.

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1
Barbara Davis Center for Childhood Diabetes, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, USA.

Abstract

Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to the use of transplantable tumors, tissue culture cell lines, and pancreatic islets as a tissue source.

PMID:
19283733
DOI:
10.1002/0471143030.cb0332s42
[Indexed for MEDLINE]
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