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Clin Cancer Res. 2009 Mar 15;15(6):2091-7. doi: 10.1158/1078-0432.CCR-08-2036. Epub 2009 Mar 10.

Fluorescence in situ hybridization analysis of circulating tumor cells in metastatic prostate cancer.

Author information

1
Molecular Cytogenetics Core Laboratory, Department of Medicine, Memorial Sloan-kettering Cancer Center, New York, New York, USA.

Abstract

PURPOSE:

To assess the feasibility of characterizing gene copy number alteration by fluorescence in situ hybridization (FISH) of circulating tumor cells (CTC) isolated using the CellSearch system in patients with progressive castration-resistant metastatic prostate cancer.

EXPERIMENTAL DESIGN:

We used probe combinations that included the androgen receptor (AR) and MYC genes for FISH analysis of CTC samples collected from 77 men with castration-resistant metastatic prostate cancer.

RESULTS:

High-level chromosomal amplification of AR was detected in 38% and relative gain of MYC in 56% of samples analyzed. No such abnormalities were detected in samples with CTC counts of <10, reflecting ascertainment difficulty in these lower count samples.

CONCLUSION:

The CTC isolated from our patient cohort present a very similar molecular cytogenetic profile to that reported for late-stage tumors and show that FISH analysis of CTC can be a valuable, noninvasive surrogate for routine tumor profiling. That as many as 50% of these patients have substantial amplification of the AR locus indicates that androgen signaling continues to play an important role in late-stage prostate cancer.

PMID:
19276271
PMCID:
PMC2875199
DOI:
10.1158/1078-0432.CCR-08-2036
[Indexed for MEDLINE]
Free PMC Article

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