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Appl Environ Microbiol. 2009 May;75(9):2936-9. doi: 10.1128/AEM.02524-08. Epub 2009 Mar 6.

PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores.

Author information

  • 1Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC 27695-7624, USA. Helen_Rawsthorne@NCSU.edu

Abstract

This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.

PMID:
19270144
PMCID:
PMC2681676
DOI:
10.1128/AEM.02524-08
[PubMed - indexed for MEDLINE]
Free PMC Article
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