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Appl Environ Microbiol. 2009 May;75(9):2964-8. doi: 10.1128/AEM.02644-08. Epub 2009 Mar 6.

Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice.

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1
Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany.

Abstract

Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.

PMID:
19270136
PMCID:
PMC2681694
DOI:
10.1128/AEM.02644-08
[Indexed for MEDLINE]
Free PMC Article
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