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Appl Environ Microbiol. 2009 May;75(9):2940-4. doi: 10.1128/AEM.01333-08. Epub 2009 Mar 6.

Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.

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  • 1Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Abstract

Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 microM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

PMID:
19270114
PMCID:
PMC2681701
DOI:
10.1128/AEM.01333-08
[PubMed - indexed for MEDLINE]
Free PMC Article
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