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J Struct Biol. 2009 Oct;168(1):95-106. doi: 10.1016/j.jsb.2009.02.013. Epub 2009 Mar 6.

Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.

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1
Department of Chemistry, University of North Carolina at Chapel Hill, 27599-3290, USA. nlt@unc.edu

Abstract

In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined.

PMID:
19269331
PMCID:
PMC2785550
DOI:
10.1016/j.jsb.2009.02.013
[Indexed for MEDLINE]
Free PMC Article
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