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Scand J Gastroenterol. 2009;44(6):664-71. doi: 10.1080/00365520902783683.

Expression of protease-activated-receptor 2 (PAR-2) in human esophageal mucosa.

Author information

1
Department of Gastrosurgical Research and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Abstract

OBJECTIVE:

The role of duodenal reflux in gastroesophageal reflux disease (GERD) containing bile salts and pancreatic enzymes (with special attention to trypsin) is still under discussion. Proteinase-activated receptors (PARs) are a novel family and PAR-2 is a unique member of this family because it is activated by trypsin. The aim of the present study was to examine the presence and the position of the PAR-2 receptor in human esophageal mucosa in different subgroups of GERD.

MATERIAL AND METHODS:

Distal biopsies taken from healthy controls, patients with erosive reflux disease (ERD), patients with specialized intestinal metaplasia (SIM) and adenocarcinoma were analyzed for the PAR-2 receptor with reverse-transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry.

RESULTS:

Gene transcripts for the PAR-2 receptor were found in all groups, with increased levels in SIM patients compared to controls. However, this visual pattern was not seen for the protein expression of the PAR-2 receptor showing no apparent quantitative differences between the groups. Immunohistochemistry revealed distinct staining for the PAR-2 receptor in the luminal part of the esophageal epithelium.

CONCLUSIONS:

The localization of the PAR-2 receptor indicates that the receptor can be cleaved and activated by trypsin in duodenogastric esophageal refluxate. The data thus suggest that the trypsin-PAR-2 pathway may be involved in the pathogenesis of GERD.

PMID:
19263271
DOI:
10.1080/00365520902783683
[Indexed for MEDLINE]

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