Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis

Vanessa Vitoriano da Silva; Cristina Dislich Ropke; Denise Varella Miranda; Rebeca Leite de Almeida; Tânia Cristina Higashi Sawada; Diogo Pineda Rivelli; Silvia Berlanga de Moraes Barros

doi:10.1080/15569520902784014

Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis

Cutan Ocul Toxicol. 2009;28(2):54-60. doi: 10.1080/15569520902784014.

Authors

Vanessa Vitoriano da Silva¹, Cristina Dislich Ropke, Denise Varella Miranda, Rebeca Leite de Almeida, Tânia Cristina Higashi Sawada, Diogo Pineda Rivelli, Silvia Berlanga de Moraes Barros

Affiliation

¹ Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brasil. vavitoriano@gmail.com

PMID: 19263261
DOI: 10.1080/15569520902784014

Abstract

In this study we assessed the protective effect of topical application of Pothomorphe umbellata extract on ultraviolet B (UVB)-induced skin lesion parameters in hairless mouse epidermis. A single dose of UVB irradiation (0.23 kJ/m2) resulted in a significant decrease in thymine dimer-positive cells and apoptotic sunburn cells, with an increase in p53 and proliferating cell nuclear antigen-positive cells in the epidermis. After 5 weeks (total dose 13.17 kJ/m2) and 15 weeks (total dose 55.51 kJ/m2) of irradiation, P. umbellata treatment inhibited the hyperplasic response and induced an increase in p53-positive cells. These findings suggest that P. umbellata extract affords protection against UVB-induced skin lesions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Cutaneous
Animals
Apoptosis / drug effects
Apoptosis / radiation effects
Biomarkers / metabolism
Carcinogenicity Tests
Epidermis / drug effects*
Epidermis / metabolism
Epidermis / pathology
Epidermis / radiation effects
Female
Hyperplasia
Immunohistochemistry
Mice
Mice, Hairless
Neoplasms, Radiation-Induced / etiology
Neoplasms, Radiation-Induced / metabolism
Neoplasms, Radiation-Induced / pathology
Neoplasms, Radiation-Induced / prevention & control*
Piperaceae / chemistry*
Plant Extracts / administration & dosage
Plant Extracts / isolation & purification
Plant Extracts / therapeutic use
Proliferating Cell Nuclear Antigen / metabolism
Pyrimidine Dimers / metabolism
Radiation-Protective Agents / administration & dosage
Radiation-Protective Agents / isolation & purification
Radiation-Protective Agents / therapeutic use*
Skin Neoplasms / etiology
Skin Neoplasms / metabolism
Skin Neoplasms / pathology
Skin Neoplasms / prevention & control*
Tumor Suppressor Protein p53 / biosynthesis
Ultraviolet Rays / adverse effects*

Substances

Biomarkers
Plant Extracts
Proliferating Cell Nuclear Antigen
Pyrimidine Dimers
Radiation-Protective Agents
Tumor Suppressor Protein p53