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Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2797-9.

[Determination of anthraquinone in Semen Cassiae from different regions by HPLC].

[Article in Chinese]

Author information

1
Chongqing Academy of Chinese Materia Medcia, Chongqing 400065, China.

Abstract

OBJECTIVE:

To determine the content of 7 anthraquinones in Semen Cassiae.

METHOD:

A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).

RESULT:

Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.

CONCLUSION:

The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.

PMID:
19260315
[Indexed for MEDLINE]

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