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BMC Microbiol. 2009 Mar 3;9:47. doi: 10.1186/1471-2180-9-47.

Rapid detection of laboratory cross-contamination with Mycobacterium tuberculosis using multispacer sequence typing.

Author information

1
Faculté de Médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236, IRD 3R198, Université de la Méditerranée, IFR 48, Marseille, France.

Abstract

BACKGROUND:

The ability to culture Mycobacterium tuberculosis from clinical specimens serves as the gold standard for the diagnosis of tuberculosis. However, a number of false-positive diagnoses may be due to cross-contamination of such specimens. We herein investigate such episode of cross-contamination by using a technique known as multispacer sequence typing (MST). This technique was applied to six M. tuberculosis isolates prepared within the same laboratory over a two-week period of time.

RESULTS:

MST analysis indicated a unique and common sequence profile between a strain isolated from a patient with proven pulmonary tuberculosis and a strain isolated from a patient diagnosed with lung carcinoma. Using this approach, we were able to provide a clear demonstration of laboratory cross-contamination within just four working days. Further epidemiological investigations revealed that the two isolates were processed for culture on the same day.

CONCLUSION:

The application of MST has been demonstrated to serve as a rapid and efficient method to investigate cases of possible cross-contamination with M. tuberculosis.

PMID:
19257895
PMCID:
PMC2653047
DOI:
10.1186/1471-2180-9-47
[Indexed for MEDLINE]
Free PMC Article

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