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Appl Microbiol Biotechnol. 2009 Jun;83(3):501-5. doi: 10.1007/s00253-009-1921-z. Epub 2009 Mar 3.

Molecular characterization of an atoxigenic Aspergillus flavus strain AF051.

Author information

1
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.

Abstract

An atoxigenic Aspergillus flavus strain AF051 collected from a peanut field in Jiangsu province, P. R. China was characterized by analysis of aflatoxin gene cluster in this study. By using a thermal asymmetric interlaced PCR (TAIL-PCR) and conventional PCR techniques, an 89.59-kb deletion was found in the cluster, and this deletion was replaced by a 3.83-kb insert, which was located at 300-bp upstream ver1 gene and 2594-bp downstream a putative gluconolactone oxidase gene. Based on the DNA sequence at the breakpoint, a nested-PCR method was developed for the rapid and sensitive detection of AF051 strain in soil and peanut samples once the strain is used as a biological agent.

PMID:
19255755
DOI:
10.1007/s00253-009-1921-z
[Indexed for MEDLINE]

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