Send to

Choose Destination
Appl Microbiol Biotechnol. 2009 Jun;83(3):501-5. doi: 10.1007/s00253-009-1921-z. Epub 2009 Mar 3.

Molecular characterization of an atoxigenic Aspergillus flavus strain AF051.

Author information

Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.


An atoxigenic Aspergillus flavus strain AF051 collected from a peanut field in Jiangsu province, P. R. China was characterized by analysis of aflatoxin gene cluster in this study. By using a thermal asymmetric interlaced PCR (TAIL-PCR) and conventional PCR techniques, an 89.59-kb deletion was found in the cluster, and this deletion was replaced by a 3.83-kb insert, which was located at 300-bp upstream ver1 gene and 2594-bp downstream a putative gluconolactone oxidase gene. Based on the DNA sequence at the breakpoint, a nested-PCR method was developed for the rapid and sensitive detection of AF051 strain in soil and peanut samples once the strain is used as a biological agent.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center