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Am J Obstet Gynecol. 2009 Mar;200(3):311.e1-311.e10. doi: 10.1016/j.ajog.2008.10.045.

Alteration of secretory leukocyte protease inhibitor in human myometrium during labor.

Author information

1
Department of Obstetrics and Gynecology, Women's and Children's Hospital, Los Angeles County-University of Southern California Medical Center, Los Angeles, CA 90033, USA. aiminli@usc.edu

Abstract

OBJECTIVE:

Secretory leukocyte protease inhibitor (SLPI) has been shown to have antimicrobial and antiinflammatory properties. The aim of this study was to verify its expression in human myometrium.

STUDY DESIGN:

Myometrium was obtained at time of cesarean delivery with (n = 9) or without (n = 11) labor. Expression of SLPI was detected using real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. SLPI expression relative to nuclear factor-kappaB p65 subunit was compared between subjects. SLPI response to inflammatory mediators was studied in myometrial explants.

RESULTS:

SLPI was predominantly localized in the nuclei of myocytes and colocalized with CD68(+) macrophages. The nuclear immunoreactivity of SLPI was increased after the onset of labor and was associated with increased nuclear translocation of nuclear factor-kappaB p65 subunit. Treatment with lipopolysaccharide, interleukin-1beta, or tumor necrosis factor-alpha increased SLPI messenger RNA and protein concentrations slightly in myometrium explants.

CONCLUSION:

SLPI was expressed in human myometrium and increased after the onset of labor.

PMID:
19254589
DOI:
10.1016/j.ajog.2008.10.045
[Indexed for MEDLINE]

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