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Biochem Biophys Res Commun. 2009 Mar 6;380(2):387-91. doi: 10.1016/j.bbrc.2009.01.100. Epub 2009 Jan 23.

Downregulation of junctional adhesion molecule-A is involved in the progression of clear cell renal cell carcinoma.

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Pharmazentrum frankfurt/ZAFES, Klinikum der, Goethe-Universit├Ąt Frankfurt, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.


Junctional adhesion molecule-A (JAM-A) is one component of tight junctions which are involved in important processes like paracellular permeability, cell polarity, adhesion, migration, and angiogenesis. Here we describe JAM-A expression in distal convoluted tubule, connecting tubule, and in cells of the collecting duct of the healthy human kidney. In addition, JAM-A was weakly expressed in cells of the proximal tubule. Using immunofluorescence, FACS and Western blot analysis we investigated JAM-A expression in tubular cells in vitro. Interestingly, treatment of HK-2 cells with IFN-gamma and TNF-alpha resulted in a metalloproteinase mediated downregulation of JAM-A. Importantly, in a tissue micro-array JAM-A protein expression was significantly downregulated in patients with clear cell renal cell carcinoma. Furthermore, knockdown of JAM-A with JAM-A specific siRNA induced the migration of RCC4 cells. In summary, downregulation of JAM-A is an early event in the development of renal cancer and increases the migration of renal cancer cells.

[Indexed for MEDLINE]

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