Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Chem Biol. 2009 Feb;13(1):3-9. doi: 10.1016/j.cbpa.2009.01.017. Epub 2009 Feb 25.

Directed enzyme evolution: climbing fitness peaks one amino acid at a time.

Author information

1
California Institute of Technology, Pasadena, 91125, USA.

Abstract

Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse. Activities on new substrates can be obtained by improving variants with broadened specificities or by step-wise evolution through a sequence of more and more challenging substrates. Evolution of highly specific enzymes has been demonstrated, even with positive selection alone. It is apparent that many solutions exist for any given problem, and there are often many paths that lead uphill, one step at a time.

Comment in

PMID:
19249235
PMCID:
PMC2703427
DOI:
10.1016/j.cbpa.2009.01.017
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center