Format

Send to

Choose Destination
BMC Microbiol. 2009 Feb 25;9:44. doi: 10.1186/1471-2180-9-44.

Quelling targets the rDNA locus and functions in rDNA copy number control.

Author information

1
Dipartimento di Biotecnologie Cellulari ed Ematologia, Università La Sapienza, Rome, Italy. gc2352@columbia.edu

Abstract

BACKGROUND:

RNA silencing occurs in a broad range of organisms. Although its ancestral function is probably related to the genome defense mechanism against repetitive selfish elements, it has been found that RNA silencing regulates different cellular processes such as gene expression and chromosomal segregation. In Neurospora crassa, a RNA silencing mechanism, called quelling, acts to repress the expression of transgenes and transposons, but until now no other cellular functions have been shown to be regulated by this mechanism.

RESULTS:

Here, we detected by northern blotting endogenous short interfering RNA (siRNAs) from the repetitive ribosomal DNA locus (rDNA) that are loaded onto the argonaute protein QDE-2. Moreover, we found a bidirectional transcription that can generate double strand RNA (dsRNA) molecules. Interestingly, quelling mutants have a reduced rDNA gene copy number.

CONCLUSION:

Our finding could suggest a new biological function for RNA silencing in the maintenance of the integrity and stability of the Neurospora rDNA locus.

PMID:
19243581
PMCID:
PMC2650698
DOI:
10.1186/1471-2180-9-44
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center