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J Am Chem Soc. 2009 Mar 25;131(11):3826-7. doi: 10.1021/ja809702n.

Silver ion unusually stabilizes the structure of a parallel-motif DNA triplex.

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1
Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. toshi@chem.kumamoto-u.ac.jp

Abstract

A simple and easy method for stabilizing DNA triplexes using Ag(+) is reported. A silver ion displaces the N3 proton of cytosine in Hoogsteen base-pairing to form the base triplet CG.CAg(+). By the addition of an equimolar amount of Ag(+), the third-strand 15-mer sequence containing five cytosines was stabilized by approximately 30 degrees C in melting temperature at pH 7. The triplex structure was stable even under weakly basic conditions.

PMID:
19243184
DOI:
10.1021/ja809702n
[Indexed for MEDLINE]
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