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J Am Chem Soc. 2009 Mar 18;131(10):3448-9. doi: 10.1021/ja809880p.

Fast two-dimensional NMR spectroscopy of high molecular weight protein assemblies.

Author information

1
Institut de Biologie Structurale Jean-Pierre Ebel CNRS-CEA-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.

Abstract

An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl (1)H-(13)C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.

PMID:
19243101
DOI:
10.1021/ja809880p
[Indexed for MEDLINE]

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