(a) Distribution of tissue specific expression in Modules 7, 18, 23, 66, 91. Module 7 is enriched for male-biased transcripts and expression in the testes and accessory glands. Module 18 is enriched for female-biased transcripts and expression in ovaries. Module 23 is enriched for transcripts affecting reproduction and gametogenesis that are highly expressed in ovaries and male accessory glands. Module 66 is enriched for transcripts in the Notch signaling pathway and nervous system development expressed in the midgut. Module 91 is enriched for transcripts affecting the function of the nervous system with high expression in the brain. (b) Modules 23 and 91 are, respectively, enriched for the Abd-b (P = 0.004) and Adf-1 (P = 0.001) transcription factor binding motifs. Abd-b has been implicated in genital disc development and Adf-1 in memory and synaptogenesis, consistent with the inferred function of genes in these modules. (c) Network representation of module 164, emphasizing the genetic correlations between adult transcripts for three transcription factors that interact during embryonic and larval development. (d) Putative functional annotation of CG15065 as a gene encoding an Immune induced Molecule (IM). Ranking all genetically variable transcripts according to their correlation to CG15065 shows that IM1 is the strongest transcriptional correlate (r = 0.74) and IM2 is the fifth strongest (r = 0.63). The protein alignments of CG15065, IM1 and IM2 are highly conserved.