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Diagn Microbiol Infect Dis. 2009 Apr;63(4):403-8. doi: 10.1016/j.diagmicrobio.2008.12.012. Epub 2009 Feb 18.

Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.

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US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.


Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.

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