Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat

Parasitol Res. 2009 Jul;105(1):35-40. doi: 10.1007/s00436-009-1358-4. Epub 2009 Feb 17.

Abstract

The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Agglutination Tests
  • Animals
  • Antibodies, Protozoan / blood*
  • Cat Diseases / diagnosis*
  • Cat Diseases / parasitology
  • Cats
  • Dog Diseases / diagnosis*
  • Dog Diseases / parasitology
  • Dogs
  • False Negative Reactions
  • Fluorescent Antibody Technique, Indirect
  • Immunoglobulin G / blood*
  • Sensitivity and Specificity
  • Toxoplasma / immunology*
  • Toxoplasmosis, Animal / diagnosis*

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G