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Biophys J. 2009 Feb 18;96(4):L32-4. doi: 10.1016/j.bpj.2008.12.3760.

Nanoelectromechanics of methylated DNA in a synthetic nanopore.

Abstract

Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.

PMID:
19217843
PMCID:
PMC2717226
DOI:
10.1016/j.bpj.2008.12.3760
[Indexed for MEDLINE]
Free PMC Article

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