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Fertil Steril. 2010 Feb;93(2):467-74. doi: 10.1016/j.fertnstert.2008.12.094. Epub 2009 Feb 12.

Vitrification of oocytes produces high pregnancy rates when carried out in fertile women.

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1
Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA.

Abstract

OBJECTIVE:

To determine the efficiency of our vitrification technique when applied in young fertile women.

DESIGN:

Clinical research and application.

SETTING:

In vitro fertilization center.

PATIENT(S):

Twenty-one women were recruited from the navy community with 19 patients finishing the study.

INTERVENTION(S):

Vitrified oocytes with use of the electron microscopic grid method were warmed 6 months after vitrification. Surviving metaphase II oocytes were microinjected for fertilization, and clinical results were evaluated.

MAIN OUTCOME MEASURE(S):

Survival, fertilization, and cleavage rate. Pregnancy and implantation rate.

RESULT(S):

Three hundred ninety-five oocytes were warmed, of which 320 oocytes (81.0%) survived. Two hundred eighty-five metaphase II oocytes were microinjected for fertilization; 206 of them (72.3%) fertilized, and 53 embryos were transferred to 19 patients (in 20 warming cycles). Twenty-four of 53 transferred embryos (45.3%) implanted as confirmed by ultrasound examination. Of the 20 transfers, 16 resulted in clinical pregnancy (80%), 3 miscarried (15%), and 13 (65%) went on to produce 20 live births, respectively. This is much higher in comparison with our previous data using supernumerary oocytes where the rates of implantation and pregnancy were 6% and 21%. Live-birth rates per warmed oocyte and per injected oocyte were 5.1% and 7.2%, respectively.

CONCLUSION(S):

High pregnancy and implantation rates were observed after 6 months of cryopreservation by vitrification when oocytes from fertile woman were used. Proper screening of candidates for oocyte cryopreservation is of crucial importance to assure a favorable pregnancy outcome.

[Indexed for MEDLINE]

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