Format

Send to

Choose Destination
J Am Soc Mass Spectrom. 2009 Aug;20(8):1391-6. doi: 10.1016/j.jasms.2009.01.005. Epub 2009 Jan 18.

Performance evaluation of a high-field Orbitrap mass analyzer.

Author information

1
Thermo Fisher Scientific, Bremen, Germany. alexander.makarov@thermofisher.com

Abstract

A new design of the Orbitrap mass analyzer is presented. Higher frequencies of ion oscillations and hence higher resolving power over fixed acquisition time are achieved by decreasing the gap between the inner and outer Orbitrap electrodes, thus providing higher field strength for a given voltage. Experimental results confirm maximum FWHM resolving power in excess of 350,000 at m/z 524 and 600,000 at m/z 195, isotopic resolution of proteins above 40 kDa, and a single-shot dynamic range of 25,000. It was also found that mass shifts in the new design depend very little on space charge inside the analyzer. This performance was achieved using higher voltages and by careful balancing of construction tolerances and operation parameters, which appeared to vary in narrower ranges of tuning than for a standard Orbitrap analyzer.

PMID:
19216090
DOI:
10.1016/j.jasms.2009.01.005
Free full text

Supplemental Content

Full text links

Icon for Springer Icon for Elsevier Science
Loading ...
Support Center